مقالات- سایر موضوعات HTLV-1
محمودی محمود، خویی علیرضا، فرزادنیا مهدی، راستین مریم
مجله دانشگاه علوم پزشکی شهید صدوقی یزد، بهار 1386؛ دوره 15، شماره 1: صفحات 93-85
مقدمه: ویروس HTLV-1 اولین رتروویروس شناخته شده انسانی و جزء خانواده انكوویروس‌ها است. ویژگی مهم این ویروس محدودیت شیوع جغرافیایی آن است و شمال خراسان یكی از مناطق آندمیك آلودگی به ویروس HTLV-1 می‌باشد. مطالعات اپیدمیولوژی و بررسی انتشار آلودگی می‌تواند در طراحی روش‌های پیشگیری مؤثر باشد. پژوهش حاضر با هدف راه اندازی فن PCRو(Polymerase Chain Reaction) برای اولین بار در ایران، برروی بافتهای پارافینه جهت بررسی آلودگی به ویروس HTLV-1 انجام شده است. این روش زمینه را برای مطالعات اپیدمیولوژی مولكولی در راستای مشخص كردن جغرافیایی و به‌ویژه تاریخی آلودگی و نیز بررسی الگوهای انتشار آن فراهم می‌آورد.
روش بررسی: این مطالعه از نوع تجربی به صورت آزمایشگاهی به‌منظور تثبیت تكنیك است. ابتدا جستجوی ژن بتااكتین با استفاده از پرایمرهای اختصاصی مربوطه بر روی نمونه‌های بافتی پارافینه از اعضای مختلف مانند كبد، طحال، پوست و گره لنفی که از بایگانی بخش آسیب‌شناسی بیمارستان امام رضا(ع) مشهد استخراج گردیده بود صورت پذیرفت. غلظت اپتیمال کلرید منیزیم 2 میلی مول و پرایمر 8 پیکو مول بود. غلظت بهینه DNA برای بلوك‌های بافتی مختلف، متفاوت بود. پس از آ ن PCR با پرایمرهای tax ،pol ،env و LTR ژنوم ویروس HTLV-1 بر روی 50 مورد بافت پارافینی گره لنفی انجام شد. و تكرارپذیری تكنیك روی بافتهای پوست و گره لنفی آلوده به HTLV-1 نشان داده شد.
نتایج : بین 50 مورد بافتهای مربوط به گره‌های لنفی در یك مورد بیمار مبتلا به لنفوم غیرهوجكینی با پنج سری پرایمر (دوسری برای قطعه Tax و یك سری برای قطعات LTR ،Env ،Pol) در نواحی مربوط به هر كدام ایجاد باند نمود. یك مورد دیگر مربوط به یك بیمار با لنفوم غیرهوجكینی تنها با دوسری پرایمر Tax مثبت بوده و با سایر پرایمرها باندی ایجاد نكرد. با احتساب مورد اول (به سبب قطعیت آلودگی به HTLV-1) درصد شیوع در بافتهای پارافینه گره‌های لنفی 2 درصد می‌باشد. در صورت محاسبه مورد دوم شیوع آلودگی به 4 درصد افزایش می‌یابد.
نتیجه گیری : مقایسه میزان آلودگی بافتی در این مطالعه با شیوع سرولوژیك نمونه‌های خون (3/2 درصد) مطالعه دیگر از نظر آماری تفاوت معنی‌داری نشان نداد و نتایج یکدیگر را تأیید کردند (P=0.883).
کلیدواژگان: واكنش زنجیره‌ای پلیمراز، ویروس HTLV-1، لنفوم، استخراج DNA
مروتی حسن
ماهنامه پزشك و آزمایشگاه، 1388؛ دوره 8، شماره 42: صفحه 13
Hossein Rahimi 1, Seyyed Abdolrahim Rezaee 2, Narges Valizade 3, Rosita Vakili 3, Houshang
Rafatpanah
Objective(s): HTLV-I and HIV virus quantification is an important marker for assessment of virus
activities. Since there is a direct relationship between the number of virus and disease progression,
HTLV-I and HIV co-infection might have an influence on the development of viral associated
diseases, thus, viral replication of these viruses and co-infection were evaluated.
Materials and Methods: In this study, 40 subjects were selected; 14 HIV infected, 20 HTLV-I infected
and 6 HTLV-I/HIV co-infected subjects. The amount of viruses was measured using qPCR TaqMan
method and CD4 and CD8 lymphocytes were assessed by flow cytometry.
Results: The mean viral load of HIV infected subjects and HTLV-I infected individuals were
134626.07±60031.07 copies/ml and 373.6±143.3 copies/104 cells, respectively. The mean HIV
viral load in co-infected group was 158947±78203.59 copies/ml which is higher than HIV infected
group. The mean proviral load of HTLV-I in co-infected group was 222.33±82.56 copies/ml which is
lower than HTLV-I infected group (P<0.05). Also, the mean white blood cell count was higher in coinfected group (5666.67±1146.49 cells/μl). However, the differences between these subjects did
not reach to a statistical significance within 95% confidence interval level (P =0.1). No significant
differences were observed regarding CD4 and CD8 positive lymphocytes between these groups.
Conclusion: HTLV-I/HIV co-infection might promote HIV replication and could reduce the HTLV-I
proviral load, in infected cells. Considering the presence of both viruses in Khorasan provinces, it
encourages researchers and health administrators to have a better understanding of co-infection
outcome.
Background: The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.
Methods: This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for
HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested
in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by
forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical
difference between the values among different categories according to SPSS program.
Results: Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for
HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%,
0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of
donors (P<0.05) ; percentage of HIV Ag/Ab was higher in donors who had blood group ” A ” and percentage of HBs
Ag was lower in donors who had blood group O. There was no significant association between Hepatitis C and syphilis infections with ABO and Rh blood groups (P>0.05).
Conclusion: Compared with neighboring countries and the international standards, prevalence of blood –borne infections is relatively low.
Keywords: HBV, HCV, HIV, Syphilis, ABO Blood groups, Rhesus (Rh), Blood donors
To date, no studies have provided data on hepatitis B virus (HBV) prevalence among asymptomatic, healthy human T-lymphotropic virus (HTLV-I) positive carriers. This sero- and molecular epidemiology study was performed on patients in the Northeast of Iran, which is an endemic area for HTLV-I infection. A total of 109 sera were collected from HTLV-I positive healthy carriers who were admitted to Ghaem Hospital, Mashhad City. All were tested for HBV serology and subsequently, real time PCR was carried out on the samples, regardless of the results of the serology. Standard PCR and direct sequencing were applied on positive samples. All cases were negative for HBsAg, Anti-HBc, and anti-HBs were positive in 34 (31.1%), and 35 (32%) individuals, respectively. There were 19 (17.4%) cases that were positive only for anti-HBs, and they had already received HBV vaccine. 16 (15%) were positive for both anti-HBs and anti-HBc, indicating a past-resolved HBV infection. 18 (16.5%) were isolated as anti-HBc, and 56 (51.3%) were negative for all HBV serological markers. Only one subject (0.9%) had detectable HBV DNA (2153 copy/ml), and assigned as being an occult HBV infection. The low prevalence of HBsAg, despite the high percentage of anti-HBc positive cases, might be related to the suppression effect of HTLV-I on surface protein expression. The low prevalence of HBV infection among HTLV-I positive healthy carriers from an endemic region, indicates that the epidemiology of HTLV-I and HBV coinfection is related to the endemicity of HBV in that region, rather than HTLV-I endemicity. J. Med. Virol. 86:1861–1867, 2014. © 2014 Wiley Periodicals, Inc.
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Computational tools are reliable alternatives to laborious work in chimeric protein design. In this study, a chimeric antigen was designed using computational techniques for simultaneous detection of anti-HTLV-I and anti-HBV in infected sera. Databases were searched for amino acid sequences of HBV/HLV-I diagnostic antigens. The immunodominant fragments were selected based on propensity scales. The diagnostic antigen was designed using these fragments. Secondary and tertiary structures were predicted and the B-cell epitopes were mapped on the surface of built model. The synthetic DNA coding antigen was sub-cloned into pGS21a expression vector. SDS-PAGE analysis showed that glutathione fused antigen was highly expressed in E. coli BL21 (DE3) cells. The recombinant antigen was purified by nickel affinity chromatography. ELISA results showed that soluble antigen could specifically react with the HTLV-I and HBV infected sera. This specific antigen could be used as suitable agent for antibody-antigen based screening tests and can help clinicians in order to perform quick and precise screening of the HBV and HTLV-I infections.
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