Sepideh Hamidi, Haniyeh Bashizadeh-Fakhar, Ali Nazemi.
Zahedan J Res Med Sci. 2018;20(5):e59961.
Background: Human T-cell lymphotropic virus type 1 (HTLV-1) is a human retrovirus that causes two distinct diseases, adult T-cell leukemia (ATL) and a progressive and degenerative myelin disorder called myelopathy associated with HTLV-1 (tropical spastic paraparesis). Similar to human immunodeficiency virus (HIV), infections caused by HTLV-1 and -2 and retroviruses are persistent and long-lasting. Less than 5% of these infections are HTLV-related illnesses, but their treatment modalities are rare and their prognosis is poor. They are often fatal. Patients with β-thalassemia major are clearly at higher risk for HTLV-1 infection due to the need for frequent blood transfusions.
Objectives: The current study aimed at detecting the molecular screening for HTLV-1 infection based on fluorescent probe in patients with β-thalassemia.
Methods: The current experimental study was conducted on 80 blood samples collected from patients with β-thalassemia major in Shahid Rajaee Hospital of Tonekabon, Iran. After DNA extraction by the Iranian kit, molecular biological system transfer (MBST) CinnaGen company (Tehran, Iran), TaqManreal-time polymerase chain reaction (RT-PCR) was employed to detect DNAHTLV-1 genome and a tax gene-specific integrated with the genome of patients’ lymphocyte was obtained.
Results: Results of TaqMan RT-PCR indicated that out of 80 patients with β-thalassemia major referred to Shahid Rajai’e Hospital, two (2.5%) had HTLV-1.
Conclusion: Compared to other molecular and serologic techniques, HTLV can be applied to detect HTLV-1 in blood banks due to its sensitivity, simplicity, higher speed, and the ability to simultaneously diagnose HTLV and lower costs than that of fluorescence molecular methods.
Keywords: HTLV-1, β-Thalassemia, Real-Time PCR